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Cytoplasmic dynein intermediate-chain isoforms with different targeting properties created by tissue-specific alternative splicing

Citation:

Nurminsky, DI, MV Nurminskaya, EV Benevolenskaya, YY Shevelyov, DL Hartl, and VA Gvozdev. 1998. “Cytoplasmic dynein intermediate-chain isoforms with different targeting properties created by tissue-specific alternative splicing.” Mol Cell Biol 18: 6816-25.

Date Published:

Nov

Abstract:

The intermediate chains (ICs) are the subunits of the cytoplasmic dynein that provide binding of the complex to cargo organelles through interaction of their N termini with dynactin. We present evidence that in Drosophila, the IC subunits are represented by at least 10 structural isoforms, created by the alternative splicing of transcripts from a unique Cdic gene. The splicing pattern is tissue specific. A constitutive set of four IC isoforms is expressed in all tissues tested; in addition, tissue-specific isoforms are found in the ovaries and nervous tissue. The structural variations between isoforms are limited to the N terminus of the IC molecule, where the interaction with dynactin takes place. This suggests differences in the dynactin-mediated organelle binding by IC isoforms. Accordingly, when transiently expressed in Drosophila Schneider-3 cells, the IC isoforms differ in their intracellular targeting properties from each other. A mechanism is proposed for the regulation of dynein binding to organelles through the changes in the content of the IC isoform pool.

Notes:

Nurminsky, D INurminskaya, M VBenevolenskaya, E VShevelyov, Y YHartl, D LGvozdev, V AengResearch Support, Non-U.S. Gov't1998/10/17Mol Cell Biol. 1998 Nov;18(11):6816-25.

Last updated on 05/20/2015