HOME / PUBLICATIONS /

Drosophila genome project: one-hit coverage in yeast artificial chromosomes

Citation:

Ajioka, JW, DA Smoller, RW Jones, JP Carulli, AE Vellek, D Garza, AJ Link, IW Duncan, and DL Hartl. 1991. “Drosophila genome project: one-hit coverage in yeast artificial chromosomes.” Chromosoma 100: 495-509.

Date Published:

Sep

Abstract:

We present a strategy for assembling a physical map of the genome of Drosophila melanogaster based on yeast artificial chromosomes (YACs). In this paper we report 500 YACs containing inserts of Drosophila DNA averaging 200 kb that have been assigned positions on the physical map by means of in situ hybridization with salivary gland chromosomes. The cloned DNA fragments have randomly sheared ends (DY clones) or ends generated by partial digestion with either NotI (N clones) or EcoRI (E clones). Relative to the euchromatic portion of the genome, the size distribution and genomic positions of the clones reveal no significant bias in the completeness or randomness of genome coverage. The 500 mapped euchromatic clones contain an aggregate of approximately 100 million base pairs of DNA, which is approximately one genome equivalent of Drosophila euchromatin.

Notes:

Ajioka, J WSmoller, D AJones, R WCarulli, J PVellek, A EGarza, DLink, A JDuncan, I WHartl, D Leng00357/PHS HHS/Research Support, U.S. Gov't, P.H.S.GERMANY1991/09/01Chromosoma. 1991 Sep;100(8):495-509.

Last updated on 05/20/2015