@article {538606,
	title = {Nonrandom location of IS1 elements in the genomes of natural isolates of Escherichia coli},
	journal = {Mol Biol Evol},
	volume = {14},
	number = {7},
	year = {1997},
	note = {<p>Boyd, E FHartl, D LengResearch Support, U.S. Gov{\textquoteright}t, P.H.S.1997/07/01Mol Biol Evol. 1997 Jul;14(7):725-32.</p>},
	month = {Jul},
	pages = {725-32},
	abstract = {<p>We have studied the spatial distribution of IS1 elements in the genomes of natural isolates comprising the ECOR reference collection of Escherichia coli. We find evidence for nonrandomness at three levels. Many pairs of IS1 elements are in much closer proximity (\&lt; 10 kb) than can be accounted for by chance. IS1 elements in close proximity were identified by long-range PCR amplification of the genomic sequence between them. Each amplified region was sequenced and its map location determined by database screening of DNA hybridization. Among the ECOR strains with at least two IS1 elements, 54\% had one or more pairs of elements separated by \&lt; 10 kb. We propose that this type of clustering is a result of "local hopping," in which we assume that a significant proportion of tranposition events leads to the insertion of a daughter IS element in the vicinity of the parental element. A second level of nonrandomness is found in strains with a modest number of IS1 elements that are mapped through the use of inverse PCR to amplify flanking genomic sequences: in these strains, the insertion sites tend to be clustered over a smaller region of chromosome than would be expected by chance. A third level of nonrandomness is observed in the composite distribution of IS elements across strains: among 20 mapped IS1 elements, none were found in the region of 48-77 minutes, a significant gap. One region of the E. coli chromosome, at 98 min, had a cluster of IS1 elements in seven ECOR strains of diverse phylogenetic origin. We deduce from sequence analysis that this pattern of distribution is a result of initial insertion in the most recent common ancestor of these strains and therefore not a hot spot of insertion. Analysis using long-range PCR with primers for IS2 and IS3 also yielded pairs of elements in close proximity, suggesting that these elements may also occasionally transpose by local hopping.</p>},
	keywords = {*DNA Transposable Elements, Chromosome Mapping, Chromosomes, Bacterial/ultrastructure, DNA, Bacterial/*genetics, Escherichia coli/*genetics/isolation \& purification, Genetic Linkage, Phylogeny},
	isbn = {0737-4038 (Print)0737-4038 (Linking)},
	author = {Boyd, E. F. and Hartl, D. L.}
}
