@article {539586,
	title = {Mutations in the mariner transposase: the D,D(35)E consensus sequence is nonfunctional},
	journal = {Proc Natl Acad Sci U S A},
	volume = {94},
	number = {4},
	year = {1997},
	note = {<p>Lohe, A RDe Aguiar, DHartl, D LengGM33741/GM/NIGMS NIH HHS/Research Support, U.S. Gov{\textquoteright}t, P.H.S.1997/02/18Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1293-7.</p>},
	month = {Feb 18},
	pages = {1293-7},
	abstract = {<p>Genetic analysis of eukaryote transposases and comparison with their prokaryote counterparts have been greatly hindered by difficulty in isolating mutations. We describe a simple eye-color screen that facilitates isolation and analysis of mutations in the mariner transposase in Drosophila melanogaster. Use of ethyl methanesulfonate and site-directed mutagenesis has identified 18 residues that are critical for in vivo excision of a target mariner element. When the mutations were examined in heterozygous mutant/nonmutant genotypes, more than half of the mutant transposase proteins were found to reduce the activity of the wild-type transposase, as assayed by the frequency of germline excision of a target element. Remarkably, transposase function is obliterated when the D,D(34)D acidic, ion-binding domain is replaced with the consensus sequence D,D(34)E found in the nematode Tc1 transposase and in many other transposases in the superfamily. A number of mutations strongly complement wild-type transposase in a dominant-negative manner, suggestive of subunit interactions in the excision reaction; these mutations are located in a small region that includes part of the D,D(34)D motif. Transposase function also is eliminated by a mutation in the inferred initiation codon and by a mutation in a putative nuclear localization signal.</p>},
	keywords = {*Consensus Sequence, Animals, Base Sequence, Crosses, Genetic, DNA Mutational Analysis, DNA Nucleotidyltransferases/*genetics, DNA Transposable Elements/*genetics, Drosophila melanogaster/enzymology/*genetics, Genetic Complementation Test, Germ Cells/enzymology, Molecular Sequence Data, Mutagenesis, Site-Directed, Peptide Fragments/genetics, Polymerase Chain Reaction, Sequence Analysis, DNA, Sequence Deletion, Transposases},
	isbn = {0027-8424 (Print)0027-8424 (Linking)},
	author = {Lohe, A. R. and De Aguiar, D. and Hartl, D. L.}
}
