@article {539791,
	title = {Cytoplasmic dynein intermediate-chain isoforms with different targeting properties created by tissue-specific alternative splicing},
	journal = {Mol Cell Biol},
	volume = {18},
	number = {11},
	year = {1998},
	note = {<p>Nurminsky, D INurminskaya, M VBenevolenskaya, E VShevelyov, Y YHartl, D LGvozdev, V AengResearch Support, Non-U.S. Gov{\textquoteright}t1998/10/17Mol Cell Biol. 1998 Nov;18(11):6816-25.</p>},
	month = {Nov},
	pages = {6816-25},
	abstract = {<p>The intermediate chains (ICs) are the subunits of the cytoplasmic dynein that provide binding of the complex to cargo organelles through interaction of their N termini with dynactin. We present evidence that in Drosophila, the IC subunits are represented by at least 10 structural isoforms, created by the alternative splicing of transcripts from a unique Cdic gene. The splicing pattern is tissue specific. A constitutive set of four IC isoforms is expressed in all tissues tested; in addition, tissue-specific isoforms are found in the ovaries and nervous tissue. The structural variations between isoforms are limited to the N terminus of the IC molecule, where the interaction with dynactin takes place. This suggests differences in the dynactin-mediated organelle binding by IC isoforms. Accordingly, when transiently expressed in Drosophila Schneider-3 cells, the IC isoforms differ in their intracellular targeting properties from each other. A mechanism is proposed for the regulation of dynein binding to organelles through the changes in the content of the IC isoform pool.</p>},
	keywords = {*Drosophila Proteins, Alternative Splicing, Amino Acid Sequence, Animals, Base Sequence, Cell Line, Cloning, Molecular, Drosophila/*metabolism, Dyneins/*chemistry, Exons/genetics, Insect Proteins/*chemistry, Introns/genetics, Microscopy, Fluorescence, Microtubule-Associated Proteins/*metabolism, Molecular Sequence Data, RNA, Messenger/metabolism, Sequence Analysis, DNA, Sequence Homology, Amino Acid},
	isbn = {0270-7306 (Print)0270-7306 (Linking)},
	author = {Nurminsky, D. I. and Nurminskaya, M. V. and Benevolenskaya, E. V. and Shevelyov, Y. Y. and Hartl, D. L. and Gvozdev, V. A.}
}
