A genome-wide view of the spectrum of spontaneous mutations in yeast


Lynch, M, W Sung, K Morris, N Coffey, CR Landry, EB Dopman, WJ Dickinson, et al. 2008. “A genome-wide view of the spectrum of spontaneous mutations in yeast.” Proc Natl Acad Sci U S A 105: 9272-7.

Date Published:

Jul 8


The mutation process ultimately defines the genetic features of all populations and, hence, has a bearing on a wide range of issues involving evolutionary genetics, inheritance, and genetic disorders, including the predisposition to cancer. Nevertheless, formidable technical barriers have constrained our understanding of the rate at which mutations arise and the molecular spectrum of their effects. Here, we report on the use of complete-genome sequencing in the characterization of spontaneously arising mutations in the yeast Saccharomyces cerevisiae. Our results confirm some findings previously obtained by indirect methods but also yield numerous unexpected findings, in particular a very high rate of point mutation and skewed distribution of base-substitution types in the mitochondrion, a very high rate of segmental duplication and deletion in the nuclear genome, and substantial deviations in the mutational profile among various model organisms.


Lynch, MichaelSung, WayMorris, KrystalynneCoffey, NicoleLandry, Christian RDopman, Erik BDickinson, W JosephOkamoto, KazufusaKulkarni, ShilpaHartl, Daniel LThomas, W KelleyengGM068465/GM/NIGMS NIH HHS/GM36827/GM/NIGMS NIH HHS/R01 GM036827/GM/NIGMS NIH HHS/Research Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov't2008/06/28 09:00Proc Natl Acad Sci U S A. 2008 Jul 8;105(27):9272-7. doi: 10.1073/pnas.0803466105. Epub 2008 Jun 26.

Last updated on 05/12/2015