Citation:
Miller, RD, DE Dykhuizen, L Green, and DL Hartl. 1984. “Specific deletion occurring in the directed evolution of 6-phosphogluconate dehydrogenase in Escherichia coli.” Genetics 108: 765-72.
Date Published:
DecAbstract:
A novel genetic change leading to increased activity of 6-phosphogluconate dehydrogenase (6PGD) in E. coli has been observed. The mutation is a deletion of approximately 0.4 kilobase pairs occurring between the structural gene of 6PGD (gnd) and one copy of an insertion element (IS5) found normally in E. coli K12 a few hundred base pairs upstream (counterclockwise) from gnd at 44 minutes on the conventional genetic map. The deletion is associated with a threefold higher activity of 6PGD and a 57% increase in the maximum growth rate when cells are grown in gluconate.
Notes:
Miller, R DDykhuizen, D EGreen, LHartl, D LengGM30201/GM/NIGMS NIH HHS/Research Support, U.S. Gov't, P.H.S.1984/12/01Genetics. 1984 Dec;108(4):765-72.