Citation:
Date Published:
Oct 4Abstract:
The detection of single-nucleotide polymorphisms in pathogenic microorganisms has normally been carried out by trial and error. Here we show that DNA hybridization with high-density oligonucleotide arrays provides rapid and convenient detection of single-nucleotide polymorphisms in Plasmodium falciparum, despite its exceptionally high adenine-thymine (AT) content (82%). A disproportionate number of polymorphisms are found in genes encoding proteins associated with the cell membrane. These genes are targets for only 22% of the oligonucleotide probes but account for 69% of the polymorphisms. Genetic variation is also enriched in subtelomeric regions, which account for 22% of the chromosome but 76% of the polymorphisms.
Notes:
Volkman, Sarah KHartl, Daniel LWirth, Dyann FNielsen, Kaare MChoi, MeheeBatalov, SergeZhou, YingyaoPlouffe, DavidLe Roch, Karine GAbagyan, RubenWinzeler, Elizabeth AengGM61351/GM/NIGMS NIH HHS/Research Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.New York, N.Y.2002/10/05 04:00Science. 2002 Oct 4;298(5591):216-8.